Aim of the study: The aim of the study was to evaluate the hypnosis in the relief of venepuncture-induced pain and anxiety in adults. Materials and methods: It was a cross-much study including 35 adult patients. Pain and anxiety were measured using visual analogue scale (VAS) after the first peripheral catheter insertion without hypnosis and after the second venous access under hypnosis. Results: Hypnosis reduced both pain intensity and anxiety, the VAS score were significantly reduced for pain; 1.6 ± 1.4 vs. 7.2 ± 1.5 (P = 0.000), and for anxiety; 2.1 ± 1.2 vs. 6.9 ± 1.8 (P = 0.000). The heart rate was also reduced ((P = 0.002). Conclusion: These results suggested that hypnosis could reduce pain and anxiety induced by venepuncture in adult.